β-内酰胺类抗生素残留受体分析法的研究进展

doi:10.11843/j.issn.0366-6964.2014.03.003

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (3): 354-362.doi: 10.11843/j.issn.0366-6964.2014.03.003

β-内酰胺类抗生素残留受体分析法的研究进展

程古月，彭娟，王玉莲，董小冰，彭大鹏，郝海红，黄玲利，袁宗辉^*

（华中农业大学国家兽药残留基准实验室（HZAU），农业部食品兽药残留检测重点实验室，武汉 430070）

收稿日期:2013-09-24 出版日期:2014-03-23 发布日期:2014-03-23
通讯作者: 袁宗辉（1958-），男，湖北天门人，教授，博士，博士生导师，主要从事兽医药理学与毒理学、兽药残留与食品安全和新兽药研发等方面的研究，Tel：027-87287186，E-mail：yuan5802@mail.hzau.edu.cn
作者简介:程古月（1982-），女，湖北武汉人，讲师，博士，主要从事抗菌兽药作用机理和微生物检测方法的研究，Tel：027-87287165-8404，E-mail：chengguyue@mail.hzau.edu.cn
基金资助:
国际科技合作项目资助计划（2011DFA32140）；中央高校基本科研业务费专项资金（2013QC002；2011PY078）

Advances in Receptor-based Screening Methods for the Detection of β-Lactam Antibiotic Residues

CHENG Gu-yue， PENG Juan， WANG Yu-lian， DONG Xiao-bing，PENG Da-peng， HAO Hai-hong， HUANG Ling-li， YUAN Zong-hui^*

(National Reference Laboratory of Veterinary Drug Residues (HZAU)， Key Laboratory for the Detection of Veterinary Drug Residues in Foods of Ministry of Agriculture，Huazhong Agricultural University， Wuhan 430070， China)

Received:2013-09-24 Online:2014-03-23 Published:2014-03-23

摘要/Abstract

摘要：

β-内酰胺类抗生素广泛用于动物细菌感染性疾病的治疗和预防，然而可食性动物产品中的抗生素残留会增加细菌耐药性的发生，危害人体健康，还会影响乳制品生产，导致经济上遭受巨大损失，因此开发快速可靠的β-内酰胺类抗生素残留检测方法对于食品安全控制非常必要。受体分析方法因其可以对一类药物进行多残留检测，具有专一性较强、灵敏度高、自动化程度高等优点，成为目前β-内酰胺类抗生素残留筛选方法研究的热点。本文将概述β-内酰胺类抗生素的受体及其制备方法，根据标记和检测手段的不同对受体分析法进行分类，包括放射免疫受体分析法、酶标记受体分析法、胶体金标记受体分析法、酶比色法和结合生物传感器的受体分析法，并介绍每类方法在β-内酰胺类抗生素残留检测中的研究进展和应用情况。

Abstract:

β-lactam antibiotics are widely used in clinical medicine.However，antibiotic residues in edible animal products are not only trigger the development of bacterial resistance，which has some adverse effects on human health，but also affect the quality of milk，resulting in economic losses.Therefore，it is necessary to develop rapid and reliable analytical methods for detection of β-lactam antibiotic residues in food safety control.The receptor-based screening method become the hotspot in the research and application of antibiotic screening methods，because of its capability of multi-residue detection of a class of drugs，and its characteristic of specificity，high sensitivity，and high degree of automation.In this article，the β-lactam receptors and their preparation are briefly introduced.The receptor-based screening methods are further classified based on the labeling and detection techniques，including radio-receptor assay，enzyme-labeling receptor assay，colloidal gold-labeling receptor assay，enzyme colorimetry，and biosensor receptor assay.Furthermore，the research progress and application of each type of receptor-based screening method in the detection of β-lactam antibiotics are reviewed in details.

中图分类号:

程古月，彭娟，王玉莲，董小冰，彭大鹏，郝海红，黄玲利，袁宗辉. β-内酰胺类抗生素残留受体分析法的研究进展[J]. 畜牧兽医学报, 2014, 45(3): 354-362.

CHENG Gu-yue， PENG Juan， WANG Yu-lian， DONG Xiao-bing，PENG Da-peng， HAO Hai-hong， HUANG Ling-li， YUAN Zong-hui. Advances in Receptor-based Screening Methods for the Detection of β-Lactam Antibiotic Residues[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 354-362.

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β-内酰胺类抗生素残留受体分析法的研究进展

Advances in Receptor-based Screening Methods for the Detection of β-Lactam Antibiotic Residues

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